01 / 4PI NANOSCOPY
A toolbox for interferometric ultra-high resolution 3D imaging through brain sections. It integrates 4Pi single-molecule nanoscopy with in situ PSF retrieval and supports the full reconstruction workflow, from channel alignment and localization to drift correction, volume alignment, image reconstruction, and morphological analysis.
02 / DEEP LOCALIZATION
Super-resolution spatiotemporal information integration is a deep learning-driven approach for precise three-dimensional localization of ultra-high-density molecules.
03 / IN SITU PSF
INSPR constructs an in situ 3D PSF directly from single-molecule data for biplane and astigmatism-based setups. Its interface covers model generation, CPU or GPU localization, drift correction, volume alignment, and super-resolution reconstruction.
04 / LIVE-CELL ANALYSIS
An accelerated ImageJ plug-in for time-series analysis in living cells. Live-SIMBA removes the dual-channel requirement through intensity-based sampling and improves computing speed with multi-core parallel processing.
05 / BAYESIAN RECONSTRUCTION
Deep learning guided Bayesian inference combines learned local and temporal features with statistical refinement for structure reconstruction from high-density, time-series fluorescence microscopy data.